EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.253 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas fluorescens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.253 | A181S | the mutant shows 110% of wild type specific activity | Pseudomonas fluorescens |
2.3.1.253 | A82T | the mutant shows wild type specific activity | Pseudomonas fluorescens |
2.3.1.253 | L136M/S243T | the mutant shows 90% of wild type specific activity | Pseudomonas fluorescens |
2.3.1.253 | L54V | the mutant shows 110% of wild type specific activity | Pseudomonas fluorescens |
2.3.1.253 | L54V/A82T | the mutant shows wild type specific activity | Pseudomonas fluorescens |
2.3.1.253 | M21T | the mutant shows 90% of wild type specific activity | Pseudomonas fluorescens |
2.3.1.253 | M21T/L54V/A82T | the mutant shows 90% of wild type specific activity | Pseudomonas fluorescens |
2.3.1.253 | M21T/L54V/A82T/A181S | the mutant shows wild type specific activity | Pseudomonas fluorescens |
2.3.1.253 | M21T/L54V/A82T/S96R/A181S | the mutant shows 110% of wild type specific activity | Pseudomonas fluorescens |
2.3.1.253 | M21T/N27D/A82T/A181S | the mutant shows 90% of wild type specific activity | Pseudomonas fluorescens |
2.3.1.253 | M21T/N27D/L54V/A82T/A181S/S243T | the mutant shows wild type specific activity | Pseudomonas fluorescens |
2.3.1.253 | M21T/N27D/L54V/A82T/L136M/A181S/S243T | the mutant shows 110% of wild type specific activity | Pseudomonas fluorescens |
2.3.1.253 | N27D | the mutant shows 90% of wild type specific activity | Pseudomonas fluorescens |
2.3.1.253 | S96R | the mutant shows 80% of wild type specific activity | Pseudomonas fluorescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.253 | 3 malonyl-CoA | Pseudomonas fluorescens | - |
phloroglucinol + 3 CO2 + 3 CoA | phloroglucinol is 1,3,5-trihydroxylbenzene | ? | |
2.3.1.253 | 3 malonyl-CoA | Pseudomonas fluorescens Pf-5 | - |
phloroglucinol + 3 CO2 + 3 CoA | phloroglucinol is 1,3,5-trihydroxylbenzene | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.253 | Pseudomonas fluorescens | - |
- |
- |
2.3.1.253 | Pseudomonas fluorescens Pf-5 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.253 | Ni-NTA column chromatography | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.253 | 3 malonyl-CoA | - |
Pseudomonas fluorescens | phloroglucinol + 3 CO2 + 3 CoA | phloroglucinol is 1,3,5-trihydroxylbenzene | ? | |
2.3.1.253 | 3 malonyl-CoA | - |
Pseudomonas fluorescens Pf-5 | phloroglucinol + 3 CO2 + 3 CoA | phloroglucinol is 1,3,5-trihydroxylbenzene | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.253 | PhlD | - |
Pseudomonas fluorescens |
2.3.1.253 | phloroglucinol synthase | - |
Pseudomonas fluorescens |